Identificaciones de peptolab

Video: https://youtu.be/Dq73nLMHHuI

Sampling location: A water sample was collected from the wall of the bathtub adjacent to the wall, in water residues among fibres and organic debris.
Date and time of collection and observation: 6 Nov 2023 at 9 AM
All images and videos are of the same population of Colpoda ciliates.

Colpoda steinii Maupas, 1883

Diagnosis of the organisms observed:
• body reniform, dorsal side convex, ventral side almost straight
• length 25–39 µm
• apical keel with ~6 ribs
• macronucleus with one, central nucleolus
• mouth opening with a right and left field of polykineties
• cilia of the left polykinetid are long, forming a “beard”
• contractile vacuole terminal
• two caudal cilia
• flexible cell
• crystalline granules are present in the cells, visible under polarised light
• feeding on bacteria
• the only ciliate species living in the specimen

References:
Colpodea (Ciliophora) (108, Fig. 53 c, 57 a-g, 58 c, d, 66 a—p, 67 a-k, 68 a-t, 69 a-z, 70 a-j, 71 a—m, Tab. 14, 15) by W.Foissner (1993)

Ciliated protozoa - an illustrated guide to the species used as biological indicators in freshwater biology by Hartmut Bick (62, Fig. 36) (1972)

Observation by Dr. Martin Kreutz https://realmicrolife.com/colpoda-steinii/

A testate amoeba from a forest fen in Wakefield, Quebec. The specimen was air-dried and imaged in a scanning electron microscope at the Canadian Museum of Nature.

The image shows two features that differentiate Galeripora from its cousin Arcella: 1) an organic coating on the outer ventral surface of the shell, obscuring the hexagonal units out of which it is composed; and 2) small pores encircling the aperture.

This is one of three related observations of ciliates: two Stentor and its parasites Sphaerophrya from one water sample. This is an observation of parasitic ciliates (at different stages).

A water sample was taken from the bank of the Vuoksi River. The sample was stored at room temperature and observed one day after collection.

Related Observations:
1st Stentor https://www.inaturalist.org/observations/195742375
2nd Stentor https://www.inaturalist.org/observations/195742590

Video: https://youtu.be/rWhk8K2WvFE

Haltidytes crassus was a relatively common species at the time of its first description. Since then - probably due to the influence of industrial agriculture - this pretty gastrotrich has become very rare.

The main characteristic of H. crassus are the five dorsal and three ventral “jumping spines”, with which the animal catapults itself out of the danger zone when threatened.

Dorsal (juvenile animal); ds: dorsal spines (5 pairs).

The long, caudal and hair-shaped last ventral pair of spines, which far overhang the body and cross each other in resting position, is conspicuous. Three ciliary rings on the head are used for swimming locomotion, while the ventral ciliary bands typical of gastrotrichs are used for gliding over the substrate.

Ventral; cr: 3 ciliary rings for locomotion; js: jump spines; cs: long caudal spines; se: seta on papillae.

The posterior palps - as in all members of the genus Hatidytes - are on distinct papillae.

Median; br: “brain”; ph: pharynx; oo: mature ovum; in: “intestine”; mf: muscle fibers that move the jumping spines.

The internal anatomy has some peculiarities. For example, distinct muscle fibers can be seen that couple to the attachment sites of the jumping spines. Adhesive glands and the associated adhesive tubes are not present in this genus.

The typical movement and behavior of the animals can be seen in the following YouTube video:

Movie about Haltidytes crassus

Mag. 400x

• A water sample was taken on 09/03/2023 from pond-edge using a 10µ dip net to enrich for microbes. Air temp. 80°F.

Short-spined Trachelomonas. Perhaps T. hispida as seen here in Fig. 42 https://www.researchgate.net/publication/314542785_Pigmented_Euglenophytes_in_a_natural_and_shallow_lake_in_the_semiarid_region_of_Paraiba_State_Brazil

Here, a population of Cinetochilum margaritaceum is presented and measurements of several of them have been taken.

A water sample was collected from the shore of Srednerogatsky Pond. The air temperature was 14°C (57.2 °F). The sample was kept at room temperature until it was assayed 6 days later.

Video: https://youtu.be/yCe9MKw1KWQ

A predatory ciliate of the family Colpodidae that feeds on the ciliates Colpoda sp.

A sample was taken from the moss on the tree bark. The sample was stored at room temperature and observed one day after collection.

Video: https://youtu.be/S5B7_vhAbJQ

A water sample was taken from the shore of Srednerogatsky Pond. The air temperature was 14°C (57.2 °F). The sample was stored at room temperature and observed 10 days after collection.

Video: https://youtu.be/7Awc1MaxZm0

A ciliate with a curious flagellum-like process at its anterior. This structure is not a true flagellum. It does not move independently, but simply dangles from the ciliate's mouth as it swims around. Its purpose is not known. In other respects, Ileonema closely resembles Trachelophyllum. Like members of that genus it is enveloped by a coat of ornate epicortical scales, visible only in the scanning electron microscope. (See Foissner's monograph on Terrestrial and Semiterrestrial Ciliates from Venezuela and Galápagos, pp. 50ff)

Ileonema dispar is the type species of the genus, which was erected in 1884 by Alfred C. Stokes. In 1922, Penard described a similar species under the name I. simplex. I think there reason to believe that I. dispar and I. simplex are the same species, viewed in different conditions. Penard observed only one specimen, although he was able to watch it for a couple of hours. Stokes found I. dispar "in abundance", and describes it as having a variable shape: "Its changes of form are...quite constant as it rests among the algal threads and lengthens the entire body into a club-shaped creature searching for food, or contracts into an egg-shape with a short, narrow neck." However, he only illustrates one specimen (a rather pudgy one!), and it is this illustration that was reproduced in Kahl, with a short description (Kahl does not seem to have found the organism himself). Stokes also observed that his Ileonema is nearly identical in overall appearance to Trachelophyllum apiculatum: a telling comment, since the latter is usually quite slender, like Penard's simplex.

Stokes describes the "flagellum" as follows: "entire flagellum one-half the length of the body, the basal half thick, obliquely grooved and presenting a twisted or cord-like appearance, slightly tapering yet suddenly constricted at the beginning of the finely filamentous distal one-half."

The "flagella" of my critters are generally shaped much as Stokes describes: quite thick at the proximal end, finely filamentous at the distal. Seeing them at low magnification, it's easy to see how he might have misinterpreted the flagellum as being twisted, or ropelike. In my observations, the flagella of some individuals, at certain levels of illumination, appear to be quite uniform from base to tip.

The Ileonema in my population are also extremely contractile, ranging in shape from plumply ovoid to gracefully attenuated and vermiform. After they've eaten, they become almost unrecognizable!

A few more specimens of Ileonema dispar, the type species of the genus, which, as far as I know, has not been recorded since it was discovered by Stokes in 1884. It is differentiated from I. simplex by its flagellar process, which, in the species Stokes describes, is in two distinct parts, thick at the base and finely filamentous at the distal end (Stokes, 1884; Penard, 1922; Kahl, 1930). This is reflected in the name of the species, "dispar" (=unequal). Stokes also describes the species as having two contractile vacuoles in the posterior, an arrangement that would be extremely odd for a trachelophyllid ciliate. In several specimens, I observed some compartmentalization in the posterior vesicle, which could easily be read as two separate vacuoles adjacent to the "anal pore." See image #4.

The flagellar process is retractable, and its appearance is quite variable. The division of this organelle into two distinct parts--which I did not see in every specimen--strikes me as a weak character. As I noted in my previous observation, I think there is some reason to think that I. simplex should be considered a junior synonym of I. dispar (or possibly a variant, or sub-species).

I caught one in fission, and have included two images of the dividers.

I also watched an individual eating a huge clump of debris containing living and dead algae. This observation, and the contents of the cytoplasm in other individuals, lead me to think the critter lives by consuming small round green algae.